渲染靶标:SSR
Render Timestamp: 2025-03-17T23:03:55.535Z
Commit: 9fc0f116116d9da247dc8ddd4e5fe811153412e1
XML generation date: 2024-09-30 01:59:04.392
Product last modified at: 2025-01-14T18:00:09.085Z
1% for the Planet 标识
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

PHOX2B (E4Q9R) Rabbit mAb #27870

Filter:
  • WB
  • IP
  • IF
Western Blotting Image 1: PHOX2B (E4Q9R) Rabbit mAb
Western blot analysis of extracts from various cell lines using PHOX2B (E4Q9R) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of PHOX2B protein in Jurkat and HeLa cells is consistent with the predicted expression pattern.


To Purchase # 27870

Cat. #

Size

Price

Inventory

27870T
20 µl
¥1,500

库存紧张

27870S
100 µl
¥4,990

无库存

Supporting Data

REACTIVITY H M
SENSITIVITY Endogenous
MW (kDa) 35
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:100
Immunofluorescence (Immunocytochemistry) 1:200 - 1:800

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

PHOX2B (E4Q9R) Rabbit mAb recognizes endogenous levels of total PHOX2B protein. Species cross-reactivity for IF-IC is human only.

Species Reactivity:

Human, Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PHOX2B protein.

Background

PHOX2B (also called neuroblastoma PHOX, NBPhox) is a homeodomain transcription factor expressed primarily in neuronal cells of the peripheral autonomic nervous system (1). Research studies in animal models have shown that PHOX2B expression is specifically required for the generation and maintenance of neuroblasts derived from the neural crest (2). PHOX2B expression has likewise been identified as a sensitive and specific marker of undifferentiated neuroblastoma tumors, including metastatic neuroblastoma cells in bone marrow (3-5). Specific mutations in the gene encoding PHOX2B have been directly linked with familial neuroblastoma cases (6) in addition to autonomic nerve disorders that can co-occur with neuroblastoma (e.g., Hirschsprung's disease, congenital central hypoventilation syndrome) (7). These findings underscore the central importance of PHOX2B to development and function of the peripheral autonomic nervous system.
For Research Use Only. Not For Use In Diagnostic Procedures.
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