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PHOX2B (E4Q9R) Rabbit mAb #27870
Filter:
- WB
- IP
- IF
Western blot analysis of extracts from various cell lines using PHOX2B (E4Q9R) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of PHOX2B protein in Jurkat and HeLa cells is consistent with the predicted expression pattern.
To Purchase # 27870
Cat. #
Size
Price
Inventory
27870T
20 µl
¥1,500
库存紧张
27870S
100 µl
¥4,990
无库存
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 35 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:200 - 1:800 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
PHOX2B (E4Q9R) Rabbit mAb recognizes endogenous levels of total PHOX2B protein. Species cross-reactivity for IF-IC is human only.
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PHOX2B protein.
Background
PHOX2B (also called neuroblastoma PHOX, NBPhox) is a homeodomain transcription factor expressed primarily in neuronal cells of the peripheral autonomic nervous system (1). Research studies in animal models have shown that PHOX2B expression is specifically required for the generation and maintenance of neuroblasts derived from the neural crest (2). PHOX2B expression has likewise been identified as a sensitive and specific marker of undifferentiated neuroblastoma tumors, including metastatic neuroblastoma cells in bone marrow (3-5). Specific mutations in the gene encoding PHOX2B have been directly linked with familial neuroblastoma cases (6) in addition to autonomic nerve disorders that can co-occur with neuroblastoma (e.g., Hirschsprung's disease, congenital central hypoventilation syndrome) (7). These findings underscore the central importance of PHOX2B to development and function of the peripheral autonomic nervous system.
- Brunet, J.F. and Pattyn, A. (2002) Curr Opin Genet Dev 12, 435-40.
- Stanke, M. et al. (1999) Development 126, 4087-94.
- Hata, J.L. et al. (2015) Arch Pathol Lab Med 139, 543-6.
- Hung, Y.P. et al. (2017) Histopathology 71, 786-794.
- Warren, M. and Shimada, H. (2016) Pediatr Dev Pathol 19, 254-5.
- Bourdeaut, F. et al. (2005) Cancer Lett 228, 51-8.
- Gaultier, C. et al. (2005) Pediatr Res 58, 1-6.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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