渲染靶标:SSR
Render Timestamp: 2025-03-16T19:26:37.949Z
Commit: 9fc0f116116d9da247dc8ddd4e5fe811153412e1
XML generation date: 2024-04-05 20:48:02.298
Product last modified at: 2025-01-01T09:01:23.578Z
1% for the Planet 标识
PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

PHOX2B Antibody #83811

Filter:
  • WB
Western Blotting Image 1: PHOX2B Antibody
Western blot analysis of extracts from various cell lines using PHOX2B Antibody (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of PHOX2B protein in Jurkat cells is consistent with the predicted expression pattern.

To Purchase # 83811

Supporting Data

REACTIVITY H M
SENSITIVITY Endogenous
MW (kDa) 35
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

PHOX2B Antibody recognizes endogenous levels of total PHOX2B protein.

Species Reactivity:

Human, Mouse

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro284 of human PHOX2B protein. Antibodies are purified by peptide affinity chromatography.

Background

PHOX2B (also called neuroblastoma PHOX, NBPhox) is a homeodomain transcription factor expressed primarily in neuronal cells of the peripheral autonomic nervous system (1). Research studies in animal models have shown that PHOX2B expression is specifically required for the generation and maintenance of neuroblasts derived from the neural crest (2). PHOX2B expression has likewise been identified as a sensitive and specific marker of undifferentiated neuroblastoma tumors, including metastatic neuroblastoma cells in bone marrow (3-5). Specific mutations in the gene encoding PHOX2B have been directly linked with familial neuroblastoma cases (6) in addition to autonomic nerve disorders that can co-occur with neuroblastoma (e.g., Hirschsprung's disease, congenital central hypoventilation syndrome) (7). These findings underscore the central importance of PHOX2B to development and function of the peripheral autonomic nervous system.
For Research Use Only. Not For Use In Diagnostic Procedures.
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