Phospho-VASP (Ser157) Antibody #3111
Filter:
- WB
Supporting Data
| REACTIVITY | H M R Mk GP |
| SENSITIVITY | Endogenous |
| MW (kDa) | 50 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
- GP-Guinea Pig
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Phospho-VASP (Ser157) Antibody detects endogenous levels of VASP only when phosphorylated at serine 157. The antibody may cross-react with the phosphorylated VASP homologue Mena.
Species Reactivity:
Human, Mouse, Rat, Monkey, Guinea Pig
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser157 of human VASP. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Vasodilator-stimulated phosphoprotein (VASP) was originally characterized as a substrate of both cGMP- and cAMP-dependent kinases (PKG and PKA, or cGPK and cAPK, respectively) (1). It is now believed that VASP belongs to the Ena/VASP family of adaptor proteins linking the cytoskeletal system to the signal transduction pathways and that it functions in cytoskeletal organization, fibroblast migration, platelet activation, and axon guidance (2,3). Three phosphorylation sites, Ser157, Ser239, and Thr278, have been identified. Ser239 is the major PKG phosphorylation site, while Ser157 is the major PKA phosphorylation site (4). Evidence suggests that VASP phosphorylation reduces its association with actin and has a negative effect on actin polymerization (5). Phosphorylation at Ser239 of VASP is a useful marker for monitoring PKG activation and signaling (6,7).
- Butt, E. et al. (1994) J Biol Chem 269, 14509-17.
- Ball, L.J. et al. (2000) EMBO J 19, 4903-14.
- Machesky, L.M. (2000) Cell 101, 685-8.
- Smolenski, A. et al. (1998) J Biol Chem 273, 20029-35.
- Harbeck, B. et al. (2000) J Biol Chem 275, 30817-25.
- Oelze, M. et al. (2000) Circ Res 87, 999-1005.
- Lawrence, D.W. and Pryzwansky, K.B. (2001) J Immunol 166, 5550-6.
限制使用
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