R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411
Filter:
- WB
- IP
- IHC
- IF
- F
Supporting Data
| REACTIVITY | All |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Mouse IgG1 |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- All-All Species Expected
Product Information
Product Usage Information
The phosphorylated form of the peptide can be detected with Phospho-Tyrosine mAb (P-Tyr-100) #9411. Sample kinase protocol is attached.
| Application | Dilution |
|---|---|
| Western Blotting | 1:2000 |
| Immunoprecipitation | 1:100 |
| Immunohistochemistry (Paraffin) | 1:2400 - 1:9600 |
| Immunofluorescence (Frozen) | 1:1600 - 1:3200 |
| Immunofluorescence (Immunocytochemistry) | 1:1600 - 1:3200 |
| Flow Cytometry (Fixed/Permeabilized) | 1:1600 - 1:6400 |
| Peptide ELISA (DELFIA) | 1:4000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #80379.
For a carrier free (BSA and azide free) version of this product see product #80379.
Protocol
Specificity / Sensitivity
Phospho-Tyrosine Mouse mAb (P-Tyr-100) is a high affinity antibody. ELISAs against a wide variety of phosphopeptides indicate that P-Tyr-100 binds phospho-Tyr in a manner largely independent of the surrounding amino acid sequence. 2D gel Western blot analysis of pervanadate-treated cell extracts also shows that P-Tyr-100 interacts with a broad range of tyrosine-phosphorylated proteins. P-Tyr-100 does not cross-react with peptides containing phospho-Ser or phospho-Thr.
Species Reactivity:
All Species Expected
Source / Purification
Monoclonal antibody is produced by immunizing animals with phospho-tyrosine containing peptides .
Background
Tyrosine phosphorylation plays a key role in cellular signaling (1). Research studies have shown that in cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology are exceptionally sensitive tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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