Phospho-Threonine/Tyrosine Antibody #9381
Filter:
- WB
- IP
Western blot analysis of extracts from Jurkat cells, untreated or calyculin A-treated (0.1 µM for 45 minutes prior to lysis) and subjected to 2-D gel electrophoresis, using Phospho-Threonine/Tyrosine Antibody.
Supporting Data
| REACTIVITY | All |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- All-All Species Expected
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Peptide ELISA (DELFIA) | 1:2000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Phospho-Threonine/Tyrosine Antibody detects proteins and peptides phosphorylated at threonine and tyrosine residues in a manner largely independent of the surrounding amino acid sequence. The antibody is phospho-specific and may cross-react slightly with some phospho-serine-containing sequences. By ELISA, it recognizes a wide variety of threonine-phosphorylated and tyrosine-phosphorylated peptides. CST recommends the use of Phospho-Threonine-Proline mAb (p-Thr-Pro-101) #9391 to detect proteins containing threonine followed by proline. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)
Species Reactivity:
All Species Expected
Source / Purification
Polyclonal antibodies are produced by immunizing animals with synthetic phospho-Thr-containing peptides. Antibodies are purified by protein A affinity chromatography.
Background
Much of the dynamic behavior of cellular proteins, including the regulation of molecular interactions (1), subcellular localization (2), and transcriptional regulation (3) is controlled by a variety of post-translational modifications (4). Antibodies specific for these post-translational modifications are invaluable tools in the quest to understand normal and pathogenic molecular and cellular behavior.
General protein modification antibodies are designed to react with modified amino acid residues (e.g. phospho-threonine, phospho-tyrosine, acetyl-lysine, nitro-tyrosine) independently of the sequence in which they are embedded. This ability to recognize modified residues in a "context-independent" fashion gives these antibodies broad reactivities, presumably conferring upon them the ability to react with hundreds of distinct proteins. This broad pattern of reactivity makes these antibodies especially valuable in multiplex analyses and target discovery programs.
Protein kinases are among the most abundant eukaryotic regulatory proteins; over 500 separate kinase genes are encoded in mammalian genomes (5,6). In spite of the importance of kinases in eukaryotic biology, relatively few of their physiological targets are known. Phospho-Threonine Antibody (P-Thr-Polyclonal) #9381 and Phospho-Threonine (42H4) mAb #9386 provide powerful tools for discovering targets of serine/threonine kinases, for monitoring and characterizing in vitro threonine phosphorylation reactions as well as for high throughput Ser/Thr kinase drug discovery.
General protein modification antibodies are designed to react with modified amino acid residues (e.g. phospho-threonine, phospho-tyrosine, acetyl-lysine, nitro-tyrosine) independently of the sequence in which they are embedded. This ability to recognize modified residues in a "context-independent" fashion gives these antibodies broad reactivities, presumably conferring upon them the ability to react with hundreds of distinct proteins. This broad pattern of reactivity makes these antibodies especially valuable in multiplex analyses and target discovery programs.
Protein kinases are among the most abundant eukaryotic regulatory proteins; over 500 separate kinase genes are encoded in mammalian genomes (5,6). In spite of the importance of kinases in eukaryotic biology, relatively few of their physiological targets are known. Phospho-Threonine Antibody (P-Thr-Polyclonal) #9381 and Phospho-Threonine (42H4) mAb #9386 provide powerful tools for discovering targets of serine/threonine kinases, for monitoring and characterizing in vitro threonine phosphorylation reactions as well as for high throughput Ser/Thr kinase drug discovery.
- Yaffe, M.B. and Elia, A.E. (2001) Curr Opin Cell Biol 13, 131-8.
- Appella, E. and Anderson, C.W. (2001) Eur J Biochem 268, 2764-72.
- Jenuwein, T. and Allis, C.D. (2001) Science 293, 1074-80.
- Krishna, R.G. and Wold, F. (1993) Adv Enzymol Relat Areas Mol Biol 67, 265-98.
- Venter, J.C. et al. (2001) Science 291, 1304-51.
- Manning, G. et al. (2002) Science 298, 1912-34.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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