R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Phospho-Thr-Pro-Pro Motif [pTPP] (D61C3) Rabbit mAb #5757
Filter:
- WB
Supporting Data
| REACTIVITY | All |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- All-All Species Expected
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Phospho-Thr-Pro-Pro Motif [pTPP] (D61C3) Rabbit mAb recognizes endogenous levels of proteins only when phosphorylated within the TPP motif. It does not cross react with phospho-serine or phospho-threonine in other contexts.
Species Reactivity:
All Species Expected
Source / Purification
Monoclonal antibody is produced by immunizing animals with synthetic peptide library containing the phospho-Thr-Pro-Pro motif.
Background
The phospho-Thr-Pro-Pro motif is a subgroup of the phospho-Thr-Pro motif that is phosphorylated by proline-directed protein kinases, including MAP kinases and cyclin dependent kinases (CDKs). Proline-directed phosphorylation is one of the major regulatory phosphorylation events in cell proliferation, cell differentiation, and a number of other essential cellular processes (1-6). This motif was identified in both phospho-proteomic and motif X analyses as a significant phospho-thr motif (7,8).
- Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.
- Seger, R. and Krebs, E.G. (1995) FASEB J 9, 726-35.
- Nurse, P. (2000) Cell 100, 71-8.
- Cross, T.G. et al. (2000) Exp Cell Res 256, 34-41.
- Yang, C.C. et al. (1998) J Protein Chem 17, 329-35.
- Reynolds, C.H. et al. (2000) J Neurochem 74, 1587-95.
- Beausoleil, S.A. et al. (2004) Proc Natl Acad Sci U S A 101, 12130-5.
- Schwartz, D. and Gygi, S.P. (2005) Nat Biotechnol 23, 1391-8.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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