R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Phospho-TFE3 (Ser321) (F1E8R) Rabbit mAb #38274
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 76-90 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Phospho-TFE3 (Ser321) (F1E8R) Rabbit mAb recognizes endogenous levels of TFE3 protein only when phosphorylated at Ser321.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser321 of human TFE3 protein.
Background
Transcription factor E3 (TFE3) is a member of a family of basic helix-loop-helix leucine zipper transcription factors that include MITF, TFEB, TFE3, and TFEC. Members of this family form heterodimers with each other, bind the same DNA sequences, and undergo the same types of post-translational modifications, including sumoylation (1). Research studies indicate that TFE3 and other family members play roles in development, organelle biogenesis, nutrient sensing, autophagy, and energy metabolism (2,3). Additional studies report that TFE3 controls the gate for pluripotent cells to exit the state of pluripotency prior to differentiation (4). Translocations involving the TFE3 gene region have been identified in a number of tumors, including sporadic renal cell tumors. Several specific translocations that result in kidney cancer and involve the TFE3 gene have been described and characterized in detail (5).
Phosphorylation of TFE3 at Ser321 by mTOR leads to cytoplasmic retention analogous to Ser211 on TFEB (6). Dephosphorylation of TFEB and TFE3 by protein phosphatase 2A in response to oxidative stress leads to their nuclear translocation and activation (7).
Phosphorylation of TFE3 at Ser321 by mTOR leads to cytoplasmic retention analogous to Ser211 on TFEB (6). Dephosphorylation of TFEB and TFE3 by protein phosphatase 2A in response to oxidative stress leads to their nuclear translocation and activation (7).
- Miller, A.J. et al. (2005) J Biol Chem 280, 146-55.
- Martina, J.A. et al. (2014) Cell Mol Life Sci 71, 2483-97.
- Martina, J.A. et al. (2014) Sci Signal 7, ra9.
- Betschinger, J. et al. (2013) Cell 153, 335-47.
- Kauffman, E.C. et al. (2014) Nat Rev Urol 11, 465-75.
- Wada, S. et al. (2016) Genes Dev 30, 2551-2564.
- Martina, J.A. and Puertollano, R. (2018) J Biol Chem 293, 12525-12534.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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