R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb (BSA and Azide Free) #54382
Filter:
- WB
- ELISA
- IF
- F
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 84 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- ELISA-ELISA
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
This product is the carrier free version of product #5483. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Formulation
Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.
For standard formulation of this product see product #5483
For standard formulation of this product see product #5483
Storage
Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.
Specificity / Sensitivity
Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb (BSA and Azide Free) detects endogenous levels of TBK1 only when phosphorylated at Ser172. This antibody may cross-react with phospho-IKKε. Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb (BSA and Azide Free) lacks sensitivity in fixed frozen mouse tissue by indirect immunofluorescent detection.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is prepared from animals immunized with a synthetic phosphopeptide corresponding to residues surrounding Ser172 of human TBK1.
Background
TBK1 (TANK-binding kinase 1)/NAK (NF-κB activating kinase) is an IκB kinase (IKK)-activating kinase and can activate IKK through direct phosphorylation (1). TBK1 was identified through association with the TRAF binding protein, TANK, and found to function upstream of NIK and IKK in the activation of NF-κB (2). TBK1 induces IκB degradation and NF-κB activity through IKKβ. TBK1 may mediate IKK and NF-κB activation in response to growth factors that stimulate PKCε activity (1). TBK1 plays a pivotal role in the activation of IRF3 in the innate immune response (3).
TBK1 is phosphorylated at Ser172 within its activation loop, which is necessary of its kinase activity including the downstream phosphorylation of IRF3 (4,5).
TBK1 is phosphorylated at Ser172 within its activation loop, which is necessary of its kinase activity including the downstream phosphorylation of IRF3 (4,5).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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