渲染靶标:SSR
Render Timestamp: 2025-04-02T13:55:11.620Z
Commit: 779953b12a5930618aae6aca7c87fb286faeb1d7
XML generation date: 2025-03-13 22:10:09.661
Product last modified at: 2025-03-18T16:30:10.299Z
1% for the Planet 标识
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Phospho-Tau (Thr205) (E7D3E) Rabbit mAb #49561

Filter:
  • WB
  • IP
  • IHC
  • IF
Western Blotting Image 1: Phospho-Tau (Thr205) (E7D3E) Rabbit mAb
Western blot analysis of normal mouse brain and Tau KO (-/-) mouse brain with Phospho-Tau (Thr205) (E7D3E) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Tau-KO mouse brain tissue was kindly provided by Dr. Dominic Walsh at Brigham and Women's Hospital and Harvard Medical School.<

To Purchase # 49561

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 50-80
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • IHC-Immunohistochemistry 
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Related Products
  • Conjugates

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:100
Immunohistochemistry (Paraffin) 1:300 - 1:1200
Immunofluorescence (Frozen) 1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier-free (BSA and azide free) version of this product see product #20739.

Protocol

Specificity / Sensitivity

Phospho-Tau (Thr205) (E7D3E) Rabbit mAb recognizes endogenous levels of Tau protein only when phosphorylated at Thr205.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the Thr205 of the human Tau protein.

Background

Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, glycogen synthase kinase-3 (GSK-3), and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease (AD); these tangles are bundles of paired helical filaments (PHFs) composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).



Phosphorylation of tau at Thr205 is found in Alzheimer's disease brain. This phosphorylation is the result of the activity of GSK-3, protein-kinase A (PKA), and other kinases (4,5). Protein phosphatases have been shown to decrease the levels of phospho-tau at Thr205, including protein phosphatase 5 (PP5) (6).
For Research Use Only. Not For Use In Diagnostic Procedures.
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