R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Phospho-Syntenin-1/MDA9 (Ser60) (F4T2K) Rabbit mAb #51623
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Transfected Only |
| MW (kDa) | 30 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Phospho-Syntenin-1/MDA9 (Ser60) (F4T2K) Rabbit mAb recognizes transfected levels of syntenin-1/MDA9 protein only when phosphorylated at Ser60.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser60 of human syntenin-1/MDA9 protein.
Background
Syntenin-1, also known as MDA9 or SDCBP, is a member of the PDZ family of proteins (1), defined by the presence of a PDZ protein interaction domain. Syntenin-1, via its PDZ domain, functions as a scaffold adaptor protein that regulates numerous signal transduction pathways, including FAK, Src, TGF-βR1, EGFR, and IGF1R (2-5). Aberrantly elevated expression of syntenin-1 has been found in various cancers and has been linked to the growth and invasion of tumor cells (5-7). In the central nervous system, syntenin-1 has been shown to mediate neuron dendrite protrusion and spine formation through its interaction with syndecan and Rheb (8). Syntenin-1 is also a key component of the syndecan/syntenin/ALIX pathway, and it has been shown to contribute directly to the formation and internalization of exosomes (9-11).
Syntenin-1/MDA9 has been shown to be phosphorylated at Ser61 by ULK1 in mouse, which is highly conserved with Ser60 in human (12).
Syntenin-1/MDA9 has been shown to be phosphorylated at Ser61 by ULK1 in mouse, which is highly conserved with Ser60 in human (12).
- Pradhan, A.K. et al. (2020) Cancer Metastasis Rev 39, 769-781.
- Dasgupta, S. et al. (2013) Clin Cancer Res 19, 4621-33.
- Das, S.K. et al. (2012) Front Biosci (Landmark Ed) 17, 1-15.
- Menezes, M.E. et al. (2016) Oncotarget 7, 80175-80189.
- Das, S.K. et al. (2018) Cancer Res 78, 2852-2863.
- Das, S.K. et al. (2019) Adv Cancer Res 144, 137-191.
- Kegelman, T.P. et al. (2015) Expert Opin Ther Targets 19, 97-112.
- Shimada, T. et al. (2019) Int J Mol Sci 20, 4171. doi: 10.3390/ijms20174171.
- Fares, J. et al. (2017) Cell Adh Migr 11, 124-126.
- Baietti, M.F. et al. (2012) Nat Cell Biol 14, 677-85.
- Kugeratski, F.G. et al. (2021) Nat Cell Biol 23, 631-641.
- Egan, D.F. et al. (2015) Mol Cell 59, 285-97.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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