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Phospho-SRPK2 (Ser497) (E5T6B) Rabbit mAb #96446
Filter:
- WB
- IP
Western blot analysis of extracts from NCI-H929, Neuro-2a, and C6 cells, untreated (-) or treated with λ phosphatase/calf intestinal phosphatase (CIP) (+), using Phospho-SRPK2 (Ser497) (E5T6B) Rabbit mAb (upper), SRPK2 (E5H7Q) Rabbit mAb #70346 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 115 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Phospho-SRPK2 (Ser497) (E5T6B) Rabbit mAb recognizes endogenous levels of SRPK2 protein only when phosphorylated at Ser497. The antibody is specific to SRPK2 (Ser497) due to the absence of an equivalent phosphorylation site in both SPRK1 and SPRK3.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser497 of human SRPK2 protein.
Background
Serine/arginine-rich protein-specific kinases (SRPKs) are a small family of serine/threonine protein kinases that phosphorylates serine residues in sequence regions containing repetitive serine/arginine-rich dipeptides (SR domains). There are three known family members (SRPK1-3), of which SRPK1 and SRPK2 are the most well-studied. Multiple research studies have shown these kinases play an important role in post-transcriptional regulation, notably via phosphorylation of SR-family RNA splicing factors (1). SRPK2 has also been implicated in lipid biogenesis, following activation through sequential phosphorylation by mTORC-activated S6K1 and casein kinase 1 (2). Phosphorylation of SRPK2 at Ser494 and Ser497 promotes nuclear translocation of SRPK2, thereby enabling its regulation of mRNA splicing factors involved in lipid biogenesis. Notably, SRPK1 and SRPK2 have both been implicated in the phosphorylation of key viral proteins (3), including the nucleocapsid protein of the SARS and SARS-CoV-2 coronaviruses (4), a modification that may be essential for viral replication.
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
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For Research Use Only. Not For Use In Diagnostic Procedures.
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