Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb (BSA and Azide Free) #34069

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Supporting Data

REACTIVITY	H M R Mk Dg
SENSITIVITY	Endogenous
MW (kDa)	60
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat
Mk-Monkey
Dg-Dog

Product Information

Product Usage Information

This product is the carrier free version of product #6943. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

Formulation

Supplied in 1X PBS (10 mM Na₂HPO₄, 3 mM KCl, 2 mM KH₂PO₄, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

For standard formulation of this product see product #6943

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb (BSA and Azide Free) detects endogenous levels of Src only when phosphorylated at Tyr416. The antibody may cross-react with other Src family members (Lyn, Fyn, Lck, Yes and Hck) when phosphorylated at equivalent sites. It may cross react with overexpressed phosphorylated RTKs.

Species Reactivity:

Human, Mouse, Rat, Monkey, Dog

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr419 of human Src protein.

Background

The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr530 in the carboxy-terminal tail by Csk renders the enzyme less active (2).

Database Links

UniProt ID: P07947 , P07948 , P06239 , P08631 , P12931 , P06241

Entrez-Gene Id: 7525 , 4067 , 3932 , 3055 , 6714 , 2534

限制使用

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For Research Use Only. Not For Use In Diagnostic Procedures.

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