R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Phospho-RCC1 (Ser11) (D18B5) Rabbit mAb #5500
Filter:
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 45 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Phospho-RCC1 (Ser11) (D18B5) Rabbit mAb recognizes endogenous levels of RCC1 protein only when phosphorylated at Ser11.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser11 of human RCC1 protein.
Background
The Ras family small GTPase Ran is involved in nuclear envelope formation, assembly of the mitotic spindle, and nuclear transport (1,2). Like other small GTPases, Ran is active in its GTP-bound form and inactive in its GDP-bound form. Nuclear RanGTP concentration is maintained through nuclear localization of guanine nucleotide exchange factor (GEF) activity, which catalyzes the exchange of bound GDP for GTP. Regulator of chromatin condensation 1 (RCC1) is the only known RanGEF (3). RCC1 is dynamically chromatin-bound throughout the cell cycle, and this localization is required for mitosis to proceed normally (4,5). Appropriate association of RCC1 with chromatin is regulated through amino-terminal phosphorylation (5,6) and methylation (7). RCC1 regulation of RanGTP levels in response to histone modifications regulates nuclear import during apoptosis (8). In mitosis RCC1 is phosphorylated at Ser11, possibly by cyclin B/cdc2 (9-11). This phosphorylation may play a role in RCC1 interaction with chromatin and RCC1 RanGEF activity (6).
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- Ohtsubo, M. et al. (1989) J Cell Biol 109, 1389-97.
- Li, H.Y. and Zheng, Y. (2004) Genes Dev 18, 512-27.
- Hutchins, J.R. et al. (2004) Curr Biol 14, 1099-104.
- Chen, T. et al. (2007) Nat Cell Biol 9, 596-603.
- Wong, C.H. et al. (2009) Nat Cell Biol 11, 36-45.
- Horiike, Y. et al. (2009) Mol Biol Rep 36, 717-23.
- Dephoure, N. et al. (2008) Proc Natl Acad Sci U S A 105, 10762-7.
- Hood, F.E. and Clarke, P.R. (2007) J Cell Sci 120, 3436-45.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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