R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Phospho-Prdx1 (Tyr194) (D1T9C) Rabbit mAb #14041
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 21 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Phospho-Prdx1 (Tyr194) (D1T9C) Rabbit mAb recognizes endogenous levels of Prdx1 protein only when phosphorylated at Tyr194. This antibody may cross react with other activated receptor tyrosine kinases including EGFR.
Species Reactivity:
Human
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr194 of human Prdx1 protein.
Background
Prdx1 belongs to a family of non-seleno peroxidases that function as H2O2 scavengers. All 6 Prdx isoforms share a conserved N-terminal cysteine (Cys51) that is oxidized by H2O2 to form cysteine-sulfenic acid (Cys51-SOH) and, in turn, reacts with Cys172-SH of another Prdx protein, forming a disulfide dimer and protecting it from degradation (1-3). Abnormally high levels of H2O2 cause Prdx1 to form an oligomeric chaperone that loses its peroxidase activity (4). Prdx family members have been reported to bind to JNK and c-Abl and regulate their kinase activity (5,6). Prdx1 was shown to bind to PTEN and regulate its phosphatase activity in conditions of mild or no cellular stress, hence preventing Akt-driven transformation by protecting PTEN from oxidation-induced inactivation (7).
The transient phosphorylation of Prdx1 at Tyr194 leads to inactivation of Prdx1 that in turn promotes localized hydrogen peroxide accumulation (8). Evidence suggests that the Tyr194 phosphorylation of Prdx1 is prominent at the wound edge during repair of cutaneous injury in mice (8,9).
The transient phosphorylation of Prdx1 at Tyr194 leads to inactivation of Prdx1 that in turn promotes localized hydrogen peroxide accumulation (8). Evidence suggests that the Tyr194 phosphorylation of Prdx1 is prominent at the wound edge during repair of cutaneous injury in mice (8,9).
- Wood, Z.A. et al. (2003) Trends Biochem Sci 28, 32-40.
- Chae, H.Z. et al. (1994) J Biol Chem 269, 27670-8.
- Chae, H.Z. et al. (1994) Proc Natl Acad Sci U S A 91, 7022-6.
- Jang, H.H. et al. (2004) Cell 117, 625-35.
- Wen, S.T. and Van Etten, R.A. (1997) Genes Dev 11, 2456-67.
- Kim, Y.J. et al. (2006) Cancer Res 66, 7136-42.
- Cao, J. et al. (2009) EMBO J 28, 1505-17.
- Woo, H.A. et al. (2010) Cell 140, 517-28.
- Singer, A.J. and Clark, R.A. (1999) N Engl J Med 341, 738-46.
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专
For Research Use Only. Not For Use In Diagnostic Procedures.
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