Phospho-PI3 Kinase Class III (Ser249) Antibody #13857
Filter:
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Transfected Only |
| MW (kDa) | 100 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Phospho-PI3 Kinase Class III (Ser249) Antibody recognizes transfected levels of PI3 kinase class III protein only when phosphorylated at Ser249.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser249 of human PI3 kinase class III protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Three distinct types of phosphoinositide 3-kinases (PI3K) have been characterized. Unlike other PI3Ks, PI3K class III catalyzes the phosphorylation of phosphatidylinositol at the D3 position, producing phosphatidylinositol-3-phosphate (PIP3) (1). PI3K class III is the mammalian homolog of Vps34, first identified in yeast. PI3K class III interacts with the regular subunit p150, the mammalian homolog of Vps15, which regulates cellular membrane association through myristoylation (2,3). PIP3 recruits several proteins with FYVE or PX domains to membranes regulating vesicular transport and protein sorting (4). Moreover, PI3K class III has been shown to regulate autophagy, trimeric G-protein signaling, and the mTOR nutrient-sensing pathway (5).
PI3 Kinase Class III/VPS34 is phosphorylated at Ser249 by the autophagy kinase ULK1 (6).
PI3 Kinase Class III/VPS34 is phosphorylated at Ser249 by the autophagy kinase ULK1 (6).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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