Phospho-PFAS (Thr619) Antibody #87933
Filter:
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 145 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Phospho-PFAS (Thr619) Antibody recognizes endogenous levels of PFAS protein only when phosphorylated at Thr619.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr619 of human PFAS protein. Antibodies are purified by peptide affinity chromatography.
Background
Phosphoribosylformylglycinamidine synthase (PFAS) is a metabolic enzyme (deamidase) associated with a protein complex known as the “purinosome,” which is involved in de novo purine synthesis (1). Research studies have shown that activation of the MAPK/ERK pathway leads to downstream activation of PFAS. Specifically, it was shown that PFAS is a substrate of ERK2, which phosphorylates PFAS at Thr619; activation of PFAS results in the de novo generation of purine intermediates required for cell and tumor growth (2). It has also been reported that both PFAS and viral PFAS-like proteins can activate RIG-I pattern recognition receptors, implicating PFAS-mediated deamidation in the innate immune response to viral infection (3).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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