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Render Timestamp: 2025-02-20T04:15:32.710Z
Commit: 1bba917eefc12d62e72a522121e2774ffbd0ee36
XML generation date: 2024-12-19 21:05:07.527
Product last modified at: 2025-01-01T09:05:47.117Z
1% for the Planet 标识
PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Phospho-PDK1 (Ser241) Antibody #3061

Filter:
  • WB
  • IP
Western Blotting Image 1: Phospho-PDK1 (Ser241) Antibody
Western blot analysis of extracts from NIH/3T3 cells (starved for 16 hours) treated with PDGF (50 ng/ml), using Phospho-PDK1 (Ser241) Antibody (upper) or control PDK1 antibody (lower). The phospho-specificity of the antibody was confirmed by treating the membrane with calf intestinal alkaline phosphatase (CIP) after Western transfer.

To Purchase # 3061

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 58-68
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Phospho-PDK1 (Ser241) Antibody detects PDK1 only when phosphorylated at Ser241.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser241 of human PDK1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Phosphoinositide-dependent protein kinase 1 (PDK1) plays a central role in many signal transduction pathways (1,2) including the activation of Akt and the PKC isoenzymes p70 S6 kinase and RSK (3). Through its effects on these kinases, PDK1 is involved in the regulation of a wide variety of processes, including cell proliferation, differentiation and apoptosis.

Several serine sites (Ser25, Ser241, Ser393/396 and Ser410) are phosphorylated on PDK1 in unstimulated human embryo kidney 293 cells, as well as IGF-1 stimulated cells (4). Phosphorylation on the activation loop Ser241 by autophosphorylation is necessary for PDK1 activity (4).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
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