Phospho-p40phox (Thr154) Antibody #4311
Filter:
- WB
Supporting Data
| REACTIVITY | H M GP |
| SENSITIVITY | Endogenous |
| MW (kDa) | 40 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- GP-Guinea Pig
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Phospho-p40phox (Thr154) Antibody detects endogenous levels of p40phox only when phosphorylated at threonine 154. This antibody does not cross-react with other phosphorylated phox subunits.
Species Reactivity:
Human, Mouse, Guinea Pig
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr154 of human p40phox. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The phagocytic NADPH oxidase is a multiprotein enzyme that catalyzes the reduction of oxygen to superoxide in response to invasion of pathogens into the body. The NADPH oxidase consists of 6 subunits, the membrane-bound p91phox and p22phox heterodimer (also known as cytochrome b558), the cytoplasmic complex of p40phox, p47phox and p67phox, and the small GTPase Rac2. Activation of NADPH oxidase is initiated by phosphorylation of the cytosolic complex, which induces comformational changes of the complex and ultimately leads to the translocation of the cytoplasmic complex to the membrane to form an active enzyme with cytochrome b558 (1). Thr154 and Ser315 of p40 phox have been identified as PKC phosphorylation sites modified during activation of the phagocyte NADPH oxidase (2).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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