渲染靶标:SSR
Render Timestamp: 2025-03-16T14:56:31.074Z
Commit: 9fc0f116116d9da247dc8ddd4e5fe811153412e1
XML generation date: 2024-09-30 01:55:09.154
Product last modified at: 2025-02-27T12:15:29.236Z
1% for the Planet 标识
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Phospho-Myosin Light Chain 2 (Ser19) Mouse mAb #3675

Filter:
  • WB
  • IF
Western Blotting Image 1: Phospho-Myosin Light Chain 2 (Ser19) Mouse mAb
Western blot analysis of extracts from HeLa cells, vehicle-treated (-) or treated with the myosin light chain kinase inhibitor ML-7 (50μM, 15 min; +) using Phospho-Myosin Light Chain 2 (Ser19) Mouse mAb (upper) or Myosin Light Chain 2 (D18E2) Rabbit mAb #8505 (lower).

To Purchase # 3675

Supporting Data

REACTIVITY H M R B Pg
SENSITIVITY Endogenous
MW (kDa) 18
Source/Isotype Mouse IgG1
Application Key:
  • WB-Western Blotting 
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • B-Bovine 
  • Pg-Pig 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Simple Western™ 1:10 - 1:50
Immunofluorescence (Immunocytochemistry) 1:200 - 1:400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Phospho-Myosin Light Chain 2 (Ser19) Mouse mAb detects endogenous levels of myosin light chain 2 (smooth muscle) only when phosphorylated at serine 19. This antibody does not cross-react with the cardiac isoform of myosin light chain 2.

Species Reactivity:

Human, Mouse, Rat, Bovine, Pig

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser19 of human myosine light chain 2 (smooth muscle).

Background

Myosin is composed of six polypeptide chains: two identical heavy chains and two pairs of light chains. Myosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC), RLC, or LC20, has many isoforms depending on its distribution. In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca2+/calmodulin-dependent manner (1). This phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction (2). ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fibers (3). Phosphorylation of smooth muscle MLC2 at Ser1/Ser2 and Ser9 by PKC and cdc2 has been reported to inhibit myosin ATPase activity (4,5). Phosphorylation by cdc2 controls the timing of cytokinesis (5). Transgenic mice lacking phosphorylation sites on the cardiac muscle isoform show morphological and functional abnormalities (6).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.