Phospho-Myelin Basic Protein (Ser295) Antibody #45316
Filter:
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 12-18 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Phospho-Myelin Basic Protein (Ser295) Antibody recognizes endogenous levels of myelin basic protein only when phosphorylated at Ser295, Ser295/Ser297, Ser295/Ser299, or Ser295/Ser297/Ser299. This antibody does not detect phosphorylation at Ser297 or Ser299 in the absence of phosphorylation at Ser295. This antibody also recognizes a non-specific band of unknown origin at 45 kDa.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser295 of human myelin basic protein. Antibodies are purified by peptide affinity chromatography.
Background
Myelin basic protein (MBP) is an abundant central nervous system (CNS) myelin membrane protein that plays an important role in nerve myelination. Myelin sheaths are multi-layered membranes derived from oligodendrocytes that increase the conduction velocity of axonal impulses. MBP helps to adhere the cytoplasmic leaflets of adjacent oligodendrocyte membranes to one another. Several splice variants of MBP are expressed in cells and the protein is modified following translation (i.e., phosphorylation, methylation), which suggests additional membrane adhesion functions (1).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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