Phospho-IKKalpha (Ser176)/IKKbeta(Ser177) Antibody #2688
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Inquiry Info. # 2688
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Supporting Data
| REACTIVITY | |
| SENSITIVITY | |
| MW (kDa) | 85 IKKalpha. 87 IKKbeta. |
| SOURCE | Rabbit |
Product Information
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity / Sensitivity
Phospho-IKKalpha (Ser176)/IKKbeta (Ser177) Antibody detects IKKalpha and IKKbeta only when phosphorylated at Ser176 or Ser177, respectively.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser176 of human IKKalpha. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation (10-13).
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限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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