Phospho-HSL (Ser660) Antibody #45804
Filter:
- WB
Western blot analysis of extracts from 3T3-L1 cells, undifferentiated (lane 1) or differentiated 4 days into adipocytes and treated with isoproterenol (10 μM, 20 min; lane 2), using Phospho-HSL (Ser660) Antibody (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 81, 83 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Phospho-HSL (Ser660) Antibody detects endogenous levels of HSL only when phosphorylated at Ser660 by PKA. This antibody does not cross-react with Ser563, Ser565, or Ser659 phosphorylated HSL.
Species Reactivity:
Human, Mouse
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser650 of human HSL (equivalent to Ser660 of rat HSL). Antibodies are purified by protein A and peptide affinity chromatography.
Background
HSL (hormone-sensitive lipase) catalyzes the hydrolysis of triacylglycerol, the rate-limiting step in lipolysis. Lipolytic stimuli activate adenylyl cyclase and thus increase intracellular cAMP levels, which in turn activate protein kinase A (PKA). PKA phosphorylates HSL at Ser563, Ser659, and Ser660, which stimulates HSL activity (1,2). In contrast, AMPK phosphorylates HSL at Ser565, which reduces HSL phosphorylation at Ser563 by PKA and inhibits HSL activity (2,3). Recent work indicates that phosphorylation at Ser600 by p44/42 MAPKs also enhances the enzymatic activity of HSL (4).
Pathways
Explore pathways related to this product.
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