Phospho-GSK-3α/β (Ser21/9) Antibody #9331
Filter:
- WB
Supporting Data
| REACTIVITY | H M R Mk Z |
| SENSITIVITY | Endogenous |
| MW (kDa) | 46 GSK-3beta. 51 GSK-3alpha. |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
- Z-Zebrafish
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Phospho-GSK-3alpha/beta (Ser21/9) Antibody detects endogenous levels of GSK-3 only when phosphorylated at serine 21 of GSK-3alpha or serine 9 of GSK-3beta.
Species Reactivity:
Human, Mouse, Rat, Monkey, Zebrafish
Source / Purification
Polyclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to the sequences of human GSK-3 alpha and beta. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).
- Welsh, G.I. et al. (1996) Trends Cell Biol 6, 274-9.
- Srivastava, A.K. and Pandey, S.K. (1998) Mol Cell Biochem 182, 135-41.
- Cross, D.A. et al. (1995) Nature 378, 785-9.
- Nusse, R. (1997) Cell 89, 321-3.
- Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511.
- Devi, L. and Ohno, M. (2015) Transl Psychiatry 5, e562.
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