Phospho-GRASP55/GORASP2 (Thr264) Antibody #53726
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- WB
Inquiry Info. # 53726
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Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 61 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Phospho-GRASP55/GORASP2 (Thr264) Antibody recognizes endogenous levels of GRASP55/GORASP2 protein only when phosphorylated at Thr264. This antibody may detect a nonspecific band around 45 kDa.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr264 of human GRASP55/GORASP2 protein. Antibodies are purified by peptide affinity chromatography.
Background
In unconventional protein secretion (UPS), cytoplasmic cargo proteins that do not have a signal peptide or a transmembrane domain are transported to the plasma membrane or extracellular matrix mainly to respond to cellular stresses (1,2). The Golgi reassembly-stacking protein 2 (GRASP55, also known as GORASP2) is essential to type III and type IV UPS (2,3). Studies show that mTORC1 phosphorylates GRASP55/GORASP2 and keeps it at the Golgi apparatus under non-stressed conditions. When cells are stressed, mTORC1 is inhibited, leading to dephosphorylation of GRASP55/GORASP2. Dephosphorylated GRASP55/GORASP2 relocates to autophagosomes and multivesicular bodies to promote UPS. In addition, Thr264 on GRASP55/GORASP2 was shown to seemingly play a key role in mTORC1-mediated phosphorylation of the protein (2).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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