Phospho-Erk5 (Thr218/Tyr220) Antibody #3371
Filter:
- WB
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Transfected Only |
| MW (kDa) | 115 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Phospho-Erk5 (Thr218/Tyr220) Antibody detects immunoprecipitated or transfected levels of Erk5 phosphorylated at threonine 218 and tyrosine 220. This antibody cross-reacts with phosphorylated Erk1 and Erk2. It does not cross-react with phosphorylated p38 MAPK or SAPK/JNK.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr218/Tyr220 of human Erk5. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Erk5 (Mitogen-activated protein kinase 7, Big mitogen-activated protein kinase 1) is a member of the MAPK superfamily implicated in the regulation numerous cellular processes including proliferation, differentiation, and survival (1-4). Like other MAPK family members, Erk5 contains a canonical activation loop TEY motif (Thr218/Tyr220) that is specifically phosphorylated by MAP2K5 (MEK5) in a growth-factor-dependent, Ras-independent mechanism (5-7). For example, EGF stimulation promotes Erk5 phosphorylation that induces its translocation to the nucleus where it phosphorylates MEF2C and other transcriptional targets (5,6). Erk5 is also activated in response to granulocyte colony-stimulating factor (G-CSF) in hematopoietic progenitor cells where it promotes survival and proliferation (7). In neuronal cells, Erk5 is required for NGF-induced neurite outgrowth, neuronal homeostasis, and survival (8,9). Erk5 is thought to play a role in blood vessel integrity via maintenance of endothelial cell migration and barrier function (10-12). Although broadly expressed, research studies have shown that mice lacking erk5 display numerous cardiac defects, suggesting Erk5 plays a critical role in vascular development and homeostasis (1,2).
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- Hayashi, M. and Lee, J.D. (2004) J Mol Med 82, 800-8.
- Wang, X. and Tournier, C. (2006) Cell Signal 18, 753-60.
- Nishimoto, S. and Nishida, E. (2006) EMBO Rep 7, 782-6.
- Kato, Y. et al. (1998) Nature 395, 713-6.
- Kato, Y. et al. (1997) EMBO J 16, 7054-66.
- Dong, F. et al. (2001) J Biol Chem 276, 10811-6.
- Obara, Y. et al. (2009) J Biol Chem 284, 23564-73.
- Finegan, K.G. et al. (2009) Cell Death Differ 16, 674-83.
- Spiering, D. et al. (2009) J Biol Chem 284, 24972-80.
- Sawhney, R.S. et al. (2009) J Cell Physiol 219, 152-61.
- Zhao, Z. et al. (2009) Mol Cell Biochem 322, 171-8.
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