Phospho-eNOS (Ser113) Antibody #9575
Filter:
- WB
Supporting Data
| REACTIVITY | H B |
| SENSITIVITY | Endogenous |
| MW (kDa) | 140 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- B-Bovine
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Phospho-eNOS (Ser113) Antibody detects endogenous levels of eNOS only when phosphorylated at serine 113.
Species Reactivity:
Human, Bovine
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser113 of human eNOS. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling, and angiogenesis (1,2). The activity of eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495 (3). Several protein kinases including Akt/PKB, PKA, and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5). In contrast, bradykinin and H2O2 activate eNOS activity by promoting both Ser1177 phosphorylation and Thr495 dephosphorylation (6,7).
eNOS is activated by VEGF, and this activation is associated with dephsophorylation of eNOS at serine 113. Cyclosporin A blocks this dephosphorylation of eNOS upon VEGF stimulation (8).
eNOS is activated by VEGF, and this activation is associated with dephsophorylation of eNOS at serine 113. Cyclosporin A blocks this dephosphorylation of eNOS upon VEGF stimulation (8).
- Fulton, D. et al. (2001) J Pharmacol Exp Ther 299, 818-24.
- Shaul, P.W. (2002) Annu Rev Physiol 64, 749-74.
- Chen, Z.P. et al. (1999) FEBS Lett 443, 285-9.
- Dimmeler, S. et al. (1999) Nature 399, 601-5.
- Fulton, D. et al. (1999) Nature 399, 597-601.
- Harris, M.B. et al. (2001) J Biol Chem 276, 16587-91.
- Thomas, S.R. et al. (2002) J Biol Chem 277, 6017-24.
- Kou, R. et al. (2002) J Biol. Chem. 277(33) , 29669-29673.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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