Phospho-eIF2α (Ser51) (E8U8L) Mouse mAb #53085
Filter:
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 38 |
| Source/Isotype | Mouse IgG1 kappa |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Phospho-eIF2α (Ser51) (E8U8L) Mouse mAb recognizes endogenous levels of eIF2α protein only when phosphorylated at Ser51. Human eIF2alpha residue Ser52 historically has been referenced as Ser51.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser52 of human eIF2α protein. Amino acid Ser52 of human eIF2α protein corresponds to amino acid Ser51 of rabbit eIF2α protein.
Background
Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. eIF2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B (1,2). Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the α subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51 (5,6).
- Kimball, S.R. (1999) Int. J. Biochem. Cell Biol. 31, 25-29.
- de Haro, C. et al. (1996) FASEB J. 10, 1378-87.
- Kaufman, R.J. (1999) Genes Dev. 13, 1211-33.
- Sheikh, M.S. and Fornace Jr., A.J. (1999) Oncogene 18, 6121-8.
- Cheshire, J.L. et al. (1999) J. Biol. Chem. 274, 4801-6.
- Zamanian-Daryoush, M. et al. (2000) Mol. Cell. Biol. 20, 1278-90.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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