Phospho-CSF-1R/M-CSF-R (Tyr546) Antibody #3083
Filter:
- WB
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Transfected Only |
| MW (kDa) | 175 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Phospho-CSF-1R/M-CSF-R (Tyr546) Antibody detects transfected levels of CSF-1R/M-CSF-R only when phosphorylated at Tyr546. This antibody may cross-react with other tyrosine-phosphorylated protein tyrosine kinases.
Species Reactivity:
Human, Mouse
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr546 of human CSF-1R/M-CSF-R. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Macrophage-colony stimulating factor (M-CSF, CSF-1) receptor is an integral membrane tyrosine kinase encoded by the c-fms proto-oncogene. M-CSF receptor is expressed in monocytes (macrophages and their progenitors) and drives growth and development of this blood cell lineage (1-3). Binding of M-CSF to its receptor induces receptor dimerization, activation, and autophosphorylation of cytoplasmic tyrosine residues used as docking sites for SH2-containing signaling proteins (4). There are at least five major tyrosine autophosphorylation sites. Tyr723 (Tyr721 in mouse) is located in the kinase insert (KI) region. Phosphorylated Tyr723 binds the p85 subunit of PI3 kinase as well as PLCγ2 (5). Phosphorylation of Tyr809 provides a docking site for Shc (5). Overactivation of this receptor can lead to a malignant phenotype in various cell systems (6). The activated M-CSF receptor has been shown to be a predictor of poor outcome in advanced epithelial ovarian carcinoma (7) and breast cancer (8).
Tyr546 is a major autophosphorylation site in the juxtamembrane domain of the M-CSF receptor. Phosphorylation of Tyr546 plays an important role in the activation of M-CSF (9) and may provide a binding site for downstream signaling components (10).
Tyr546 is a major autophosphorylation site in the juxtamembrane domain of the M-CSF receptor. Phosphorylation of Tyr546 plays an important role in the activation of M-CSF (9) and may provide a binding site for downstream signaling components (10).
- Stanley, E.R. et al. (1978) Nature 274, 168-70.
- Byrne, P.V. et al. (1981) J Cell Biol 91, 848-53.
- Bourette, R.P. and Rohrschneider, L.R. (2000) Growth Factors 17, 155-66.
- Novak, U. et al. (1996) Oncogene 13, 2607-13.
- Bourette, R.P. et al. (1997) EMBO J 16, 5880-93.
- Morley, G.M. et al. (1999) Oncogene 18, 3076-84.
- Toy, E.P. et al. (2001) Gynecol Oncol 80, 194-200.
- Maher, M.G. et al. (1998) Clin Cancer Res 4, 1851-6.
- Schubert, C. et al. (2007) J. Biol. Chem. 282, 4094-4101.
- Joos, H. et al. (1996) J. Biol. Chem. 271, 24476-24481.
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