渲染靶标:SSR
Render Timestamp:
4/4/2025, 2:01:08 AM EDT
4/4/2025, 6:01:08 AM UTC
Commit: 461ca8d8fe5b1efd4c01fc87e5b5eb592e2d154a
XML generation date: 2025-03-07 13:12:12.831
Product last modified at: 2025-03-19T14:30:10.266Z
1% for the Planet 标识
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Phospho-CREB (Ser133) (1B6) Mouse mAb #9196

Filter:
  • WB
Western Blotting Image 1: Phospho-CREB (Ser133) (1B6) Mouse mAb
Western blot analysis of extracts from SK-N-MC cells, untreated or treated with forskolin and FGF(lanes 1 and 2) or 293 cells. untreated or UV treated (lanes 3 and 4), using Phospho-CREB (Ser133) (1B6) Mouse mAb.

To Purchase # 9196

Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 43
Source/Isotype Mouse IgG1
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #80864.

Protocol

Specificity / Sensitivity

Phospho-CREB (Ser133) (1B6) Mouse mAb detects endogenous levels of CREB only when phosphorylated at serine 133. This antibody also detects the phosphorylated form of the CREB-related protein, ATF-1.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser133 of human CREB.

Background

CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways, including Erk, Ca2+, and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
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