Render Target: SSR
Render Timestamp: 2024-11-14T22:57:31.177Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-09-30 01:55:18.990
Product last modified at: 2024-09-30T08:02:05.244Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Phospho-CASC5 (Thr943/Thr1155) (D8D4N) Rabbit mAb #40758

Filter:
  • WB
  • IF

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 265
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Immunocytochemistry) 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #72586.

    Protocol

    Specificity / Sensitivity

    Phospho-CASC5 (Thr943/Thr1155) (D8D4N) Rabbit mAb recognizes endogenous levels of CASC5 protein only when phosphorylated at Thr943 or Thr1155. The protein sequences surrounding these two sites are identical.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr943 of human CASC5 protein.

    Background

    Kinetochores are mitotic structures that form on centromeres and attach to mitotic spindle microtubules. Kinetochore attachment to microtubules regulates chromosome segregation and progression through mitosis. Unattached kinetochores signal to the spindle assembly checkpoint (SAC) machinery, arresting cells in mitosis (1). CASC5, also known as Knl1 or Blinkin, is the largest subunit of the Knl1–Mis12–Ndc80 complex (KMN) network, a structural component of kinetochores required for microtubule binding. CASC5 functions in the formation of kinetochore–microtubule attachments, chromosome segregation, and in activating the SAC. CASC5 has been implicated in human diseases, including leukemia and microcephaly (2). Activation of the SAC is regulated in part by mitotic phosphorylation of CASC5 at several sites, including Ser24, Ser60, Thr943, and Thr1155 (3,4). The sequences surrounding Thr943 and Thr1155 are identical.
    For Research Use Only. Not For Use In Diagnostic Procedures.
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