Phospho-BCL2L13 (Ser275) Antibody #39233
Filter:
- WB
Western blot analysis of extracts from THP-1 cells, differentiated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (50 ng/mL, 18 hr and then rested 48 hr) followed by vehicle (-) or treated with Lipopolysaccharides (LPS) #14011 (5 μg/mL, 15 min; +), using Phospho-BCL2L13 (Ser275) Antibody (upper), BCL2L13 (E6G1Q) Rabbit mAb #61974 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 90 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Phospho-BCL2L13 (Ser275) Antibody recognizes endogenous levels of BCL2L13 protein only when phosphorylated at Ser275 of human BCL2L13 (equivalent to Ser272 of mouse BCL2L13).
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser275 of human BCL2L13 protein. Antibodies are purified by peptide affinity chromatography.
Background
BCL2L13 (Bcl-2-like protein 13, Bcl-rambo) is an atypical member of the Bcl-2 family of proteins that regulate apoptosis at the mitochondria (1). While it contains conserved Bcl-2 homology (BH) domains -1, -2, -3, and -4, it has a unique C-terminal region and does not interact with other Bcl-2 family members (1). BCL2L13 may regulate apoptosis in a cellular context manner (reviewed in 2). It was originally identified as a pro-apoptotic protein with activity dependent on its C-terminal region (1). Interaction with the adenine nucleotide translocator (ANT) suppresses the ADP/ATP-dependent translocation activity of ANT (3). Other studies have found that BCL2L13 can inhibit apoptosis in glioblastoma and tumor growth (4). These studies found that BCL2L13 binds to pro-apoptotic ceramide synthases 2 (CerS2) and 6 (CerS6) and blocks homo- and heteromeric CerS2/6 complex formation and activity. Studies have also found that BCL2L13 can regulate mitochondrial fragmentation and can bind to LC3 to facilitate the clearance of damaged mitochondria through mitophagy (5,6).
Phosphorylation of BCL2L13 at Ser275 plays a role in the clearance of damaged mitochondria through mitophagy and is important in maintaining cardiac function (7).
Phosphorylation of BCL2L13 at Ser275 plays a role in the clearance of damaged mitochondria through mitophagy and is important in maintaining cardiac function (7).
- Kataoka, T. et al. (2001) J Biol Chem 276, 19548-54.
- Kataoka, T. (2022) Front Cell Dev Biol 10, 1065702.
- Kim, J.Y. et al. (2012) FEBS Lett 586, 3142-9.
- Jensen, S.A. et al. (2014) Proc Natl Acad Sci USA 111, 5682-7.
- Murakawa, T. et al. (2015) Nat Commun 6, 7527.
- Otsu, K. et al. (2015) Autophagy 11, 1932-3.
- Murakawa, T. et al. (2024) Cell Rep 43, 115001.
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