Phospho-α-Synuclein (Ser129) (D1R1R) Rabbit mAb (BSA and Azide Free) #87281

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Supporting Data

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)	18
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IF-Immunofluorescence

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Usage Information

This product is the carrier-free version of product #23706. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

Formulation

Supplied in 1X PBS (10 mM Na₂HPO₄, 3 mM KCl, 2 mM KH₂PO₄, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

For standard formulation of this product see product #23706.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

Phospho-α-Synuclein (Ser129) (D1R1R) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of α-Synuclein protein only when phosphorylated at Ser129. Specificity of nuclear staining is unclear but consistent with previous publications (4).

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser129 of human α-Synuclein protein.

Background

α-Synuclein is a protein of 140-amino acids expressed abundantly in the brain. α-Synuclein is also the main component of pathogenic Lewy bodies and Lewy neurites. Research studies have shown that mutations of the α-Synuclein gene are linked to Parkinson's disease (1).

Various research studies have shown that phosphorylation of α-Synuclein at Ser129 is a highly toxic event causing degeneration of dopaminergic neurons associated with Parkinson's disease. This is proposed to occur through increased misfolding, aggregation, and accumulation of α-Synuclein phosphorylated at this site (2). GSK-3β is one of several kinases that has been reported to phosphorylate α-Synuclein at Ser129 (3).

Database Links

UniProt ID: P37840

Entrez-Gene Id: 6622

限制使用

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For Research Use Only. Not For Use In Diagnostic Procedures.

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