PHB1 Antibody #2426
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 29 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
PHB1 Antibody detects endogenous levels of total PHB1 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser252 of human PHB1. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The prohibitins PHB1 and PHB2 are highly conserved, multifunctional proteins present in eukaryotic nuclear and mitochondrial compartments. PHB1 is a 30 kDa tumor suppressor protein involved in cell cycle control (1). PHB1 has been found in mitochondria, the nucleus, and the plasma membrane, as well as extracellularly in circulation (2). In mitochondria, prohibitins mainly exist as membrane-bound ring complexes and function as chaperones maintaining mitochondrial protein stability during protein synthesis and transportation (3,4). In the nucleus, prohibitins interact with transcription factors such as Rb and p53 to regulate target gene transcription (2,5). Extracellular prohibitin can bind and activate C3 to enhance complement activation (6).
- McClung, J.K. et al. (1995) Exp Gerontol 30, 99-124.
- Mishra, S. et al. (2006) J. Cell. Mol. Med. 10, 353-363.
- Nijtmans, L.G. et al. (2000) EMBO J. 19, 2444-2451.
- Tatsuta, T. et al. (2005) Mol. Biol. Cell 16, 248-259.
- Fusaro, G. et al. (2003) J. Biol. Chem. 278, 47853-47861.
- Mishra, S. et al. (2007) Mol. Immunol. 44, 1907-1912.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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