PGK1 Antibody #68540
Filter:
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 43 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
PGK1 Antibody recognizes endogenous levels of total PGK1 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr298 of human PGK1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
PGK1 (phosphoglycerate kinase) is an essential enzyme in the glycolysis pathway (1). It catalyzes the reversible phospho-transfer reaction from 1,3-diphosphoglycerate to ADP to form ATP and 3-phosphoglycerate. The expression of PGK1 is upregulated in many cancer types and plays an important role in cancer cell proliferation and metastasis (2-5). PGK1 can also function as a protein kinase. ERK can phosphorylate PGK1 at Ser203. This phosphorylation changes PGK1 conformation and leads to its translocation from cytoplasm to mitochondria. There it interacts and phosphorylates PDHK1 (pyruvate dehydrogenase kinase isomerase 1), which leads to PDHK1 inhibition, blocks pyruvate to coenzyme A conversion, and promotes cytosolic lactate concentration (6). Acetylated PGK1 can phosphorylate Beclin1 to induce autophagy (7). PGK1 is activated by autophosphorylation at Tyr324. PTEN can dephosphorylate PGK1 at this site to downregulate its activity. In cancer cells, loss of PTEN enhances PGK1 activity and promotes glycolysis and tumor growth (8).
- Beutler, E. (2007) Br J Haematol 136, 3-11.
- Wilson, R.B. et al. (2019) Pleura Peritoneum 4, 20190003.
- Yu, T. et al. (2017) Cancer Res 77, 5782-94.
- Hu, H. et al. (2017) Hepatology 65, 515-28.
- Cao, H. et al. (2017) Cancer Chemother Pharmacol 79, 985-94.
- Li, X. et al. (2016) Mol Cell 61, 705-19.
- Qian, X. et al. (2017) Mol Cell 65, 917-931.e6.
- Qian, X. et al. (2019) Mol Cell, pii: S1097-2765(19)30622-7. doi: 10.1016/j.molcel.2019.08.006.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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