PFKP Antibody #5412
Filter:
- WB
- IP
Western blot analysis of extracts from various cell types using PFKP Antibody.
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 80 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
PFKP Antibody detects endogenous levels of total PFKP protein.
Species Reactivity:
Human, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PFKP protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Phosphofructokinase (PFK) catalyzes the phosphorylation of fructose-6-phosphate in glycolysis (1). There are three isozymes: muscle-type, liver-type, and platelet-type (2,3). Platelet-type phosphofructokinase (PFKP) is expressed in various cell types (4,5). Research studies have shown that genetic variations in PFKP are associated with individuals born small for gestational age that are prone to obesity and diabetes later in adulthood (6).
- Mediavilla, D. et al. (2008) J Biochem 144, 235-44.
- Eto, K. et al. (1994) Biochem Biophys Res Commun 198, 990-8.
- Hannemann, A. et al. (2005) Gene 345, 237-47.
- Morrison, N. et al. (1992) Hum Genet 89, 105-6.
- Vora, S. (1983) Isozymes Curr Top Biol Med Res 11, 3-23.
- Morgan, A.R. et al. (2010) BMC Med Genet 11, 125.
Pathways
Explore pathways related to this product.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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