R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
PDE4B (D7R1Y) Rabbit mAb #72096
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 90 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
PDE4B (D7R1Y) Rabbit mAb recognizes endogenous levels of total PDE4B protein. This antibody does not cross-react with PDE4A protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu61 of human PDE4B protein.
Background
Enzymes of the phosphodiesterase (PDE) superfamily catalyze the hydrolysis of 3',5'-cyclic nucleotides into the corresponding nucleotide 5'-monophosphates. The PDE superfamily includes 11 subfamilies (PDE1-PDE11) in mammals (1). These enzymes function as important positive and negative regulators of cellular response, including regulation of insulin secretion, heart function, erectile function, and inflammation (2-5). The cAMP-specific phosphodiesterase 4B (PDE4B, DPDE4) is important for the inflammatory response to lipopolysaccharide in monocytes (6). PDE4B plays an important role in the hydrolysis and inactivation of the ubiquitous second messenger cAMP that regulates lymphocyte cell growth and apoptosis (7). Research studies indicate that PDE4B is also involved in psychiatric disorders, including schizophrenia, autism, and depression (8-10).
- Bender, A.T. and Beavo, J.A. (2006) Pharmacol Rev 58, 488-520.
- Dyachok, O. et al. (2006) Nature 439, 349-52.
- Fischmeister, R. et al. (2006) Circ Res 99, 816-28.
- Rosen, R.C. and Kostis, J.B. (2003) Am J Cardiol 92, 9M-18M.
- Smith, S.J. et al. (2004) Mol Pharmacol 66, 1679-89.
- Jin, S.L. and Conti, M. (2002) Proc Natl Acad Sci U S A 99, 7628-33.
- Conti, M. et al. (2003) J Biol Chem 278, 5493-6.
- Fatemi, S.H. et al. (2008) Schizophr Res 101, 36-49.
- Braun, N.N. et al. (2007) Neuroreport 18, 1841-4.
- Numata, S. et al. (2009) Am J Med Genet B Neuropsychiatr Genet 150B, 527-34.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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