R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
PD-1 (Intracellular Domain) (D4W2J) XP® Rabbit mAb #86163
Filter:
- WB
- IP
- IHC
- IF
- F
Supporting Data
REACTIVITY | H |
SENSITIVITY | Endogenous |
MW (kDa) | 52-65 |
Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:200 |
IHC Leica Bond | 1:100 - 1:400 |
Immunohistochemistry (Paraffin) | 1:100 - 1:400 |
Immunofluorescence (Immunocytochemistry) | 1:200 - 1:400 |
Flow Cytometry (Fixed/Permeabilized) | 1:100 - 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #63815.
For a carrier free (BSA and azide free) version of this product see product #63815.
Protocol
Specificity / Sensitivity
PD-1 (Intracellular Domain) (D4W2J) XP® Rabbit mAb recognizes endogenous levels of total PD-1 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala274 of human PD-1 protein.
Background
The programmed cell death 1 protein (PD-1, PDCD1, CD279) is a member of the CD28 family of immunoreceptors that regulate T cell activation and immune responses (1-3). The PD-1 protein contains an extracellular Ig V domain, a transmembrane domain, and a cytoplasmic tail that includes an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an immunoreceptor tyrosine-based switch motif (ITSM). PD-1 is activated by the cell surface ligands PD-L1 and PD-L2 (4). Upon activation, PD-1 ITIM and ITSM phosphorylation leads to the recruitment of the protein tyrosine phosphatases SHP-1 and SHP-2, which suppress TCR signaling (5-7). In addition to activated T cells, PD-1 is expressed in activated B cells and monocytes, although its function in these cell types has not been fully characterized (8). The PD-1 pathway plays an important role in immune tolerance (3); however, research studies show that cancer cells often adopt this pathway to escape immune surveillance (9). Consequently, blockade of PD-1 and its ligands is proving to be a sound strategy for neoplastic intervention (10).
- Ishida, Y. et al. (1992) EMBO J 11, 3887-95.
- Shinohara, T. et al. (1994) Genomics 23, 704-6.
- Nishimura, H. et al. (1999) Immunity 11, 141-51.
- Freeman, G.J. et al. (2000) J Exp Med 192, 1027-34.
- Yokosuka, T. et al. (2012) J Exp Med 209, 1201-17.
- Sheppard, K.A. et al. (2004) FEBS Lett 574, 37-41.
- Chemnitz, J.M. et al. (2004) J Immunol 173, 945-54.
- Thibult, M.L. et al. (2013) Int Immunol 25, 129-37.
- Dong, H. et al. (2002) Nat Med 8, 793-800.
- Topalian, S.L. et al. (2012) Curr Opin Immunol 24, 207-12.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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