R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
PARG (D4E6X) Rabbit mAb #66564
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 130 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
PARG (D4E6X) Rabbit mAb recognizes endogenous levels of total PARG protein. This antibody specifically recognizes PARG isoform 1 (UniProt #Q86W56-1) and does not recognize other PARG isoforms.
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PARG protein.
Background
Poly (ADP-ribose) glycohydrolase (PARG) is an enzyme that hydrolyzes poly (ADP-ribose) (PAR) formed by members of the PAR polymerase (PARP) enzyme family. Poly (ADP)-ribosylation is a post-translational modification that is catalyzed by PARP proteins. This modification involves polymerization of ADP-ribose from NAD+ to target proteins, such as histones and transcription factors, and plays a wide range of biological roles, including the response to DNA damage and transcriptional regulation (1,2). The mammalian PARG enzyme that catalyzes the removal of this modification exists as multiple isoforms. PARG isoforms 1-3 shuttle between the nucleus and cytoplasm and are responsible for most of the PARG activity. The smaller isoforms 4 and 5 reside in the cytoplasm (3-5). Research studies link altered PAR metabolism to inflammatory and autoimmune diseases, as well as neuronal degeneration (6-8). PARG inhibitors that increase PAR levels may sensitize cells to cancer treatments (e.g., cisplatin) and may help in the development of cancer therapies (9).
- Thomas, C. and Tulin, A.V. (2013) Mol Aspects Med 34, 1124-37.
- Li, N. and Chen, J. (2014) Mol Cells 37, 9-16.
- Meyer-Ficca, M.L. et al. (2004) Exp Cell Res 297, 521-32.
- Meyer-Ficca, M.L. et al. (2005) Int J Biochem Cell Biol 37, 920-6.
- Bonicalzi, M.E. et al. (2003) Biol Cell 95, 635-44.
- Cortes, U. et al. (2004) Mol Cell Biol 24, 7163-78.
- Masutani, M. et al. (2005) Cell Mol Life Sci 62, 769-83.
- Ying, W. and Swanson, R.A. (2000) Neuroreport 11, 1385-8.
- Fauzee, N.J. et al. (2010) Pathol Oncol Res 16, 469-78.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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