R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
PAR1 (E9J9L) Rabbit mAb #79109
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 47, 70-110 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
PAR1 (E9J9L) Rabbit mAb recognizes endogenous levels of total PAR1 protein. The antibody recognizes a background band at 24 kDa of unknown origin.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp50 of human PAR1 protein.
Background
PAR1 (F2R) belongs to the PAR (Protease-Activated Receptor) family of G protein-coupled receptors. These membrane receptors are activated through amino terminal cleavage of the receptor by serine proteases such as thrombin, trypsin, or matrix metalloproteinases (1,2). Protease-mediated cleavage exposes the ‘tethered-ligand’ fragment of the receptor; this binds to a second extracellular loop of the receptor and triggers receptor activation. Activated PAR1 couples to distinct G-protein complexes or arrestins, further relaying signals to downstream players (e.g., phospholipase C, Rho-kinase) (3-4). PAR1 function is necessary for normal physiological processes such as hemostasis, platelet activation, and thrombosis, whereas abnormal activation of PAR1 has been associated with inflammatory disorders, cardiovascular diseases, and metastasis (4-6).
- Macfarlane, S.R. et al. (2001) Pharmacol Rev 53, 245-82.
- Ossovskaya, V.S. and Bunnett, N.W. (2004) Physiol Rev 84, 579-621.
- Willis Fox, O. and Preston, R.J.S. (2020) J Thromb Haemost 18, 6-16.
- Flaumenhaft, R. and De Ceunynck, K. (2017) Trends Pharmacol Sci 38, 701-16.
- Coughlin, S.R. (2005) J Thromb Haemost 3, 1800-14.
- Covic, L. and Kuliopulos, A. (2018) Int J Mol Sci 19, 2237.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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