R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
PADI4 (F1B9L) Rabbit mAb #76469
Filter:
- WB
- IP
- IF
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 68 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:200 - 1:800 |
| Flow Cytometry (Fixed/Permeabilized) | 1:50 - 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
PADI4 (F1B9L) Rabbit mAb recognizes endogenous levels of total PADI4 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant human PADI4 protein.
Background
Peptidyl arginine deiminase (PAD) proteins are a family of Ca2+-dependent enzymes that catalyze the post-translational conversion of arginine to citrulline. There are currently five known PAD isozymes in humans, referred to as PADI1-4 and PADI6 (1). PADI4 is a nuclear member of the PAD family and is responsible for deiminating R2, R8, R17, and R26 on the histone H3 (2-4). Deimination of histone residues prevents CARM1-mediated arginine methylation and results in transcriptional repression (2,3). Expression of PADI4 is increased during myeloid differentiation and various polymorphisms have been implicated in rheumatoid arthritis (5-8). PADI4 can play a role in apoptosis and cell cycle arrest upon DNA damage, as it is recruited to the p21 promoter by p53 and also regulates a subset of p53 target genes (9,10). Hypercitrullination of histones by PADI4 contributes to chromatin decondensation and the formation of neutrophil extracellular traps (NETs) to combat bacterial infection in a process called NETosis (11,12). Inhibition of PADI4 reduces NET formation, making it a potential therapeutic target (13).
- Jones, J.E. et al. (2009) Curr Opin Drug Discov Devel 12, 616-27.
- Cuthbert, G.L. et al. (2004) Cell 118, 545-53.
- Wang, Y. et al. (2004) Science 306, 279-83.
- Nakashima, K. et al. (2002) J Biol Chem 277, 49562-8.
- Nakashima, K. et al. (1999) J Biol Chem 274, 27786-92.
- Suzuki, A. et al. (2003) Nat Genet 34, 395-402.
- Worthington, J. and John, S. (2003) Trends Mol Med 9, 405-7.
- Yamada, R. et al. (2003) Trends Mol Med 9, 503-8.
- Li, P. et al. (2008) Mol Cell Biol 28, 4745-58.
- Yao, H. et al. (2008) J Biol Chem 283, 20060-8.
- Wang, Y. et al. (2009) J Cell Biol 184, 205-13.
- Brinkmann, V. et al. (2004) Science 303, 1532-5.
- Lewis, H.D. et al. (2015) Nat Chem Biol 11, 189-91.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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