Render Target: SSR
Render Timestamp: 2025-03-06T19:20:04.011Z
Commit: 9fc0f116116d9da247dc8ddd4e5fe811153412e1
XML generation date: 2024-09-30 01:59:26.512
Product last modified at: 2025-02-27T13:00:14.269Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

P4HA1 (E3I3R) Rabbit mAb #61182

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 60
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    P4HA1 (E3I3R) Rabbit mAb recognizes endogenous levels of total P4HA1 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro358 of human P4HA1 isoform 2 protein.

    Background

    Prolyl 4-hydroxylase subunit alpha-1 (P4HA1) is a member of the prolyl 4-hydroxylase enzyme family, which is responsible for catalyzing the hydroxylation of specific proline residues within collagen polypeptide chains. This hydroxylation is a crucial step in collagen synthesis and maturation. The hydroxylated proline residues are essential for the stabilization of collagen's triple helical structure by facilitating the formation of hydrogen bonds. This structural stability is fundamental to collagen's mechanical properties and its ability to function as a structural scaffold in various tissues (1). The hydroxylation of proline residues in collagen is also critical for subsequent crosslinking reactions, which further strengthen the collagen fibrils and contribute to the overall stability of tissues. These crosslinks are important for tissue integrity, wound healing, and overall physiological function (1). P4HA1 has recently been identified in gene signatures associated with several tumors (2,3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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