R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
p400 (E8R5S) Rabbit mAb #83054
Filter:
- WB
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 400 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
p400 (E8R5S) Rabbit mAb recognizes endogenous levels of total p400 protein.
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1003 of human p400 protein.
Background
p400, also known as EP400 or Domino, is a chromatin remodeler initially found to interact with the adenovirus oncoprotein E1A (1,2). p400 is part of the TIP60 histone acetyltransferase complex and is an ATPase capable of catalyzing the exchange of histone H2A with the variant histone H2AZ (3,4). This unique role of p400 has been shown to affect important processes by modifying gene expression through changes in chromatin structure. p400 plays a role in Hox gene expression during embryonic hematopoiesis (5). Also, p400 can regulate p21 to control cell cycle and senescence (6,7). p400 is recruited to sites of double-stranded breaks to destabilize nucleosomes, resulting in RNF8-mediated chromatin ubiquitination and recruitment of BRCA1, 53BP1, and Rad51 (8,9). The activity of p400 is important in the development and survival of Schwann cells and oligodendrocytes (10,11). In addition to H2A/H2AZ exchange, p400 can also exchange H3 with the variant H3.3 to contribute to gene regulation (12).
- Fuchs, M. et al. (2001) Cell 106, 297-307.
- Ogawa, H. et al. (2003) Genes Cells 8, 325-39.
- Mizuguchi, G. et al. (2004) Science 303, 343-8.
- Kusch, T. et al. (2004) Science 306, 2084-7.
- Ueda, T. et al. (2007) Genes Cells 12, 581-92.
- Chan, H.M. et al. (2005) Genes Dev 19, 196-201.
- Gévry, N. et al. (2007) Genes Dev 21, 1869-81.
- Xu, Y. et al. (2010) J Cell Biol 191, 31-43.
- Courilleau, C. et al. (2012) J Cell Biol 199, 1067-81.
- Fröb, F. et al. (2019) Nat Commun 10, 2361.
- Elsesser, O. et al. (2019) Nucleic Acids Res 47, 6208-6224.
- Pradhan, S.K. et al. (2016) Mol Cell 61, 27-38.
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our
Trademark Information page.