渲染靶标:SSR
Render Timestamp: 2025-03-16T12:57:20.420Z
Commit: 9fc0f116116d9da247dc8ddd4e5fe811153412e1
XML generation date: 2024-09-30 01:57:57.718
Product last modified at: 2025-02-11T20:30:11.500Z
1% for the Planet 标识
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

p19 ARF (E9X4Z) Rabbit mAb #77184

Filter:
  • WB
Western Blotting Image 1: p19 ARF (E9X4Z) Rabbit mAb
Western blot analysis of extracts from various mouse cell lines using p19 ARF (E9X4Z) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of p19 ARF protein in NIH/3T3 cells is consistent with the predicted expression pattern.

To Purchase # 77184

Supporting Data

REACTIVITY M
SENSITIVITY Endogenous
MW (kDa) 21
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • M-Mouse 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

p19 ARF (E9X4Z) Rabbit mAb recognizes endogenous levels of total p19 ARF protein. This antibody does not recognize p16 INK4A protein or human p14 ARF protein.

Species Reactivity:

Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu92 of mouse p19 ARF protein.

Background

Human p14 ARF (p19 ARF in mouse) is a pro-apoptotic cell cycle regulator frequently inactive in human tumors (1). Basal expression of p14 ARF is low in most cell types, but accumulation of this protein occurs in response to oncogene expression (2,3). Increased p14 ARF levels facilitate MDM2 degradation, leading to increased p53 protein levels and subsequent cell cycle arrest and/or apoptosis (4). While most p14 ARF signaling has traditionally thought to be p53-dependent, more recent reports have described p53-independent p14 ARF signaling leading to cell cycle arrest and apoptosis (5,6).
For Research Use Only. Not For Use In Diagnostic Procedures.
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