R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
ORF1p (D3W9O) Rabbit mAb #88701
Filter:
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 42 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
ORF1p (D3W9O) Rabbit mAb recognizes endogenous levels of total ORF1p protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly162 of human ORF1p protein.
Background
LINE-1, also known as L1, is a non-long terminal repeat (non-LTR) retrotransposon with hundreds of thousands of copies in the human genome (1, 2). Like all non-LTRs, L1 replicates by target-primed reverse transcription (TPRT) (3). The L1 retrotransposon encodes two proteins critical to this process: ORF1p and ORF2p. ORF2p contributes to endonuclease and reverse transcriptase activity, while ORF1p acts as a nucleic acid chaperone that binds RNA (4-8). Many types of cancers have been shown to have L1 insertions within tumor suppressor genes, disrupting their expression and contributing to tumorigenesis (9).
While normally silenced, ORF1p is expressed in many types of cancers (9-12). Recently, it has been found that ORF1p can become phosphorylated and that this event impacts its ability to promote retrotransposition. This creates a novel dynamic to which the host could actively affect L1 replication through various signaling cascades (13).
While normally silenced, ORF1p is expressed in many types of cancers (9-12). Recently, it has been found that ORF1p can become phosphorylated and that this event impacts its ability to promote retrotransposition. This creates a novel dynamic to which the host could actively affect L1 replication through various signaling cascades (13).
- Kazazian, H.H. and Moran, J.V. (1998) Nat Genet 19, 19-24.
- Malik, H.S. et al. (1999) Mol Biol Evol 16, 793-805.
- Wilhelm, M. and Wilhelm, F.X. (2001) Cell Mol Life Sci 58, 1246-62.
- Scott, A.F. et al. (1987) Genomics 1, 113-25.
- Moran, J.V. et al. (1996) Cell 87, 917-27.
- Feng, Q. et al. (1996) Cell 87, 905-16.
- Martin, S.L. and Bushman, F.D. (2001) Mol Cell Biol 21, 467-75.
- Kolosha, V.O. and Martin, S.L. (1997) Proc Natl Acad Sci U S A 94, 10155-60.
- Lee, E. et al. (2012) Science 337, 967-71.
- Rodić, N. et al. (2015) Nat Med 21, 1060-4.
- Doucet-O'Hare, T.T. et al. (2015) Proc Natl Acad Sci U S A 112, E4894-900.
- Chen, L. et al. (2012) Breast Cancer Res Treat 136, 129-42.
- Rodić, N. et al. (2014) Am J Pathol 184, 1280-6.
- Cook, P.R. et al. (2015) Proc Natl Acad Sci U S A 112, 4298-303.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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