Olig2 (E6G6Q) XP^® Rabbit mAb (BSA and Azide Free) #71735

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Supporting Data

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)	35
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IHC-Immunohistochemistry
IF-Immunofluorescence

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Usage Information

This product is the carrier-free version of product #65915. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

Formulation

Supplied in 1X PBS (10 mM Na₂HPO₄, 3 mM KCl, 2 mM KH₂PO₄, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

For standard formulation of this product see product #65915.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

Olig2 (E6G6Q) XP^® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total Olig2 protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human Olig2 protein.

Background

Olig2 is a basic helix–loop–helix (bHLH) transcription factor necessary for oligodendrocyte and motor neuron differentiation and development in the central nervous system (1,2). Olig2 has been shown to play a role in Purkinje cell specification in the early cerebellum (3). Olig2 has also been shown to be a useful marker for primary and mature oligodendrocytes and is universally expressed in diffuse gliomas (4,5).

Database Links

UniProt ID: Q13516

Entrez-Gene Id: 10215

限制使用

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Olig2 (E6G6Q) XP® Rabbit mAb (BSA and Azide Free) #71735