OGDH Antibody #13407
Inquiry Info. # 13407
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Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 110 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
OGDH Antibody recognizes endogenous levels of total OGDH protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala110 of mouse OGDH protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
2-oxoglutarate dehydrogenase (OGDH) is one of three enzymes in the α ketoglutarate dehydrogenase complex (OGDC) that is responsible for catalyzing a rate-regulating step of the tricarboxylic acid (Krebs) cycle. Together with dihydrolipoamide S-succinyltransferase (DLST) and dihydrolipoamide dehydrogenase (DLD), OGDH helps to convert 2-oxoglutarate to succinyl-CoA and CO2 within eukaryotic mitochondria (1). Regulation of this enzyme complex is important for mitochondrial energy metabolism within cells (2). Research studies indicate that OGDH activity within the mitochondrial matrix is regulated by multiple factors, including calcium, the adenine nucleotides ATP and ADP, and NADH (2).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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