Oct-1 Antibody #4428
Filter:
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 90 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Oct-1 Antibody detects endogenous levels of total Oct-1 protein.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Leu362 of human Oct-1. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Oct-1 (POU2F1) is a ubiquitously expressed, octamer-binding transcription factor containing a POU domain with a homeobox subdomain (1). Oct-1 has been shown to interact with several transcription factors in mediating specific gene expression, including SNAPc (2), OBF-1 (a B-cell transcriptional coactivator protein) (3), TFIIB (4), and TBP (TATA-box-binding protein) (5). Its POU DNA-binding domain allows Oct-1 the flexibility to facilitate the binding and recruitment of several tissue-specific cofactors to either positively or negatively regulate a variety of genes, exerting an important role in development (6).
- Sturm, R.A. et al. (1988) Genes Dev 2, 1582-99.
- Mittal, V. et al. (1996) Mol Cell Biol 16, 1955-65.
- Strubin, M. et al. (1995) Cell 80, 497-506.
- Nakshatri, H. et al. (1995) J Biol Chem 270, 19613-23.
- Zwilling, S. et al. (1994) Nucleic Acids Res 22, 1655-62.
- Schonemann, M.D. et al. (1998) Adv Exp Med Biol 449, 39-53.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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