R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
OCA-T2 (F5A7Z) Rabbit mAb #48122
Filter:
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 35 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
OCA-T2 (F5A7Z) Rabbit mAb recognizes endogenous levels of total OCA-T2 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to full-length human OCA-T2 protein.
Background
OCA-T2 (COLCA2, POU2AF3) and its paralog OCA-T1 (C11orf53) were first identified by single-cell RNA sequencing (scRNAseq) to be co-expressed with the transcription factor POU2F3, in specialized epithelial cells called tuft cells (1). Tuft cells exist as solitary chemosensory cells within mucosal epithelium and serve to coordinate interactions between immune and neuronal cells (2). Systematic gene knockdown experiments demonstrated chromatin-level interactions between OCA-T1, OCA-T2, and POU2F3 that appeared essential for tuft cell lineage determination (1). Notably, the oncogenic transformation of tuft cells has been identified as a defining feature of a distinct small cell lung cancer (SCLC) subtype known as SCLC-P, defined by their dependence on POU2F3 expression (3). In vitro studies showed that targeted knockdown of OCA-T1 and OCA-T2 suppressed growth of cultured SCLC-P cell lines, supporting a role for OCA-T1 and OCA-T2 in SCLC (4). Subsequently, it has been observed that gene fusions between EWSR1 or FUS and OCA-T2 are defining features of recently discovered aggressive sarcoma subtypes (5-7).
- Wu, X.S. et al. (2022) Nature 607, 169-175.
- Schneider, C. et al. (2019) Nat Rev Immunol 19, 584-593.
- Huang, Y.H. et al. (2018) Genes Dev 32, 915-928.
- Zhou, C. et al. (2022) Cell Discov 8, 112.
- Hiemenz, M.C. et al. (2023) Genes Chromosomes Cancer 62, 460-470.
- Habenbacher, M. et al. (2024) Histopathology 85, 365-368.
- Koshyk, O. et al. (2023) Mod Pathol 36, 100337.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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