R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
OAS2 (E2G4K) Rabbit mAb #24344
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 70 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
OAS2 (E2G4K) Rabbit mAb recognizes endogenous levels of total OAS2 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala330 of human OAS2 protein.
Background
2’-5’-oligoadenylate synthetase 2 (OAS2) is an enzyme induced by type 1 interferon that plays a key role in the host antiviral innate immune response (1,2). The OAS family of proteins includes OAS1, OAS2, OAS3, and OASL in humans (3). OAS2 is activated by cytosolic dsRNA and its enzymatic activity generates the second messenger 2’-5’-linked oligoadenylate. These 2’-5’-linked oligoadenylates bind to the ribonuclease RNase L, which then degrades viral and cellular RNA in an effort to curb viral replication (1). Research studies have shown that components of the OAS-RNase L system, including OAS2, are highly upregulated in response to SARS-CoV-2 infection, suggesting a role for this antiviral pathway in the pathogenesis of SARS-CoV-2 (4,5).
- Sarkar, S.N. et al. (1999) J Biol Chem 274, 25535-42.
- Sarkar, S.N. et al. (1999) J Biol Chem 274, 1848-55.
- Choi, U.Y. et al. (2015) Exp Mol Med 47, e144.
- Li, Y. et al. (2020) bioRxiv, 2020.09.24.312553. doi: 10.1101/2020.09.24.312553.
- Schmidt, N. et al. (2020) Nat Microbiol, doi: 10.1038/s41564-020-00846-z.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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