OAS/RNase L Pathway Antibody Sampler Kit #60125
Product Information
Kit Usage Information
Protocols
- 7074: Western Blotting
- 14498: Western Blotting, Immunoprecipitation (Agarose)
- 24344: Western Blotting, Immunoprecipitation (Agarose)
- 27281: Western Blotting
- 36845: Western Blotting
- 74906: Western Blotting
- 88499: Western Blotting
Product Description
The OAS/RNase L Pathway Antibody Sampler Kit provides an economical means of detecting proteins involved in the OAS/RNase L pathway. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.
Background
The 2'-5'-oligoadenylate synthetase (OAS)/RNase L pathway is a component of the antiviral innate immune system. The OAS family of proteins includes OAS1, OAS2, OAS3, and OASL in humans, all of which are induced in response to type 1 interferons (1-3). When activated by cytosolic double-stranded RNA, OAS1-3 generate 2’-5’-linked oligoadenylates that bind to and activate the ribonuclease, RNase L. Activated RNase L is poised to degrade viral and cellular RNA as part of the antiviral innate immune response (4). Unlike OAS1-3, OASL lacks intrinsic enzymatic activity, and research studies have suggested that OASL participates in the antiviral immune response independently of the canonical OAS/RNase L signaling pathway (5,6). Negative regulation of the OAS/RNase L pathway is governed, in part, by the PDE12 phosphodiesterase that degrades 2'-5'-oligoadenylates (7).
- Chebath, J. et al. Nature 330, 587-8.
- Schoggins, J.W. et al. (2011) Nature 472, 481-5.
- (2019) Proc Natl Acad Sci U S A 116, 12573.
- Dong, B. and Silverman, R.H. (1997) J Biol Chem 272, 22236-42.
- Marques, J. et al. (2008) J Gen Virol 89, 2767-2772.
- Ishibashi, M. et al. (2010) Biochem Biophys Res Commun 392, 397-402.
- Wood, E.R. et al. (2015) J Biol Chem 290, 19681-96.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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